Laboratory Research Technician I

A full-time Research Tech I position is available in Dr. Liyun Zhang’s laboratory in the Department of Ophthalmology at the University of Pittsburgh. The lab focuses on studying the mechanisms of retinal degeneration and regeneration, with the ultimate goal of developing novel therapies for retinal degenerative diseases. Using CRISPR/Cas9 technology, the lab develops zebrafish retinal disease models to investigate molecular and cellular mechanisms of photoreceptor degeneration and perform in vivo phenotypic drug screening to identify potential therapeutic compounds.

Qualification of candidate:

1. A bachelor’s degree in biology, Molecular Biology or related scientific field is required.2) Previous research experience is preferred but not mandatory, especially with the following techniques and skills:

a. Zebrafish husbandry (e.g. general care, breeding, genotyping, phenotyping, drug treatments).

b. High resolution imaging (e.g. fluorescence and confocal microscopy).

c. Histological and immunological tissue processing and sectioning.

d. Standard molecular biology techniques (e.g. PCR, cloning, RNA/DNA extraction)

3. Strong attention to details and excellent organizational skills.

4. Ability to work both independently and collaboratively within a diverse research team.

5. Enthusiasm for science and a proactive mindset to learn new techniques and contribute to innovative research.

Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering.

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Specific duties & responsibilities: The Research Technician will support the lab’s research activities by conducting experiments, maintaining zebrafish lines, and overseeing general laboratory operations to ensure the smooth day-to-day functioning of the lab. Key responsibilities include, but are not limited to:

1. Perform a variety of experiments, such as drug screening, histology, confocal imaging, zebrafish behavior assays, and microinjection. Assist with data analysis and troubleshooting.

2. Maintain detailed, accurate, and timely records of experimental data in laboratory records. Document and analyze results and contribute to lab meetings and scientific discussions.

3. Maintain zebrafish stocks and zebrafish databases, monitor fish health, and perform genotyping.

4. Sterilize experimental materials, prepare solutions, maintain a clean and safe working environment.

5. Manage laboratory supplies, including ordering, tracking, receiving, and organizing materials and reagents.

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Must have good stereo vison to effectively use a stereo microscope for larval fish dissection and related tasks.

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The University of Pittsburgh is an equal opportunity employer / disability / veteran.

Job Details Job Description: Med-Ophthalmology, Pennsylvania-Pittsburgh Assignment Category: Full-time regular Job Classification: Staff.Laboratory Research Technician I Job Family: Research Job Sub-Family: Laboratory Campus: Pittsburgh Minimum Education Level Required: Bachelor's Degree Minimum Years of Experience Required: No experience required Will this position accept substitution in lieu of education or experience?: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement. Work Schedule: M- F 8:30am to 5:00pm Work Arrangement: On-Campus: Teams that work on campus, in an office, or in a lab. Hiring Range: TBD Based Upon Qualifications Relocation Offered: No Visa Sponsorship Provided: No Background Check: For position finalists, employment with the University will require successful completion of a background check Child Protection Clearances: Not Applicable Required Documents: Resume Optional Documents: Not Applicable